anti hk2  (Boster Bio)

Journal: Scientific Reports

Article Title: LncRNA NEAT1 regulation of the miR-101-3p/RAC1 axis affects cervical cancer aerobic glycolysis and progression

doi: 10.1038/s41598-025-01698-5

Figure Lengend Snippet: LncRNA NEAT1 regulation of the miR-101-3p/RAC1 axis affects cervical cancer aerobic glycolysis and progression. ( A ) Correlation analysis plot between NEAT1 expression levels and the HALLMARK_GLYCOLYSIS pathway. ( B ) Graphical representation of GO functional annotations for genes within the HALLMARK_GLYCOLYSIS pathway significantly associated with NEAT1. ( C ) Western blotting and ( D ) qRT-PCR were employed to protein abundance and messenger RNA levels of genes associated with glycolysis, respectively, of glycolysis-related genes (HK2, PFKP, PKM2, GLUT1, LDHA) and the downstream target gene RAC1 in lung tissues (n = 3). ( E , F and G ) Quantification of glucose uptake, lactic acid production levels, and ATP generation in the lungs of nude mice were measured using relevant kits (n = 6). ( H ) FISH detection of miR-101-3p in nude mouse lungs (n = 3). ( I ) IHC examination of the downstream target gene RAC1(n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: HK2 , 1:1000 , Cell signaling technology , 2867T.

Techniques: Expressing, Functional Assay, Western Blot, Quantitative RT-PCR, Quantitative Proteomics